Effects of RNA interference targeting STAT3 gene on proliferation and apoptosis of SKOV3 cells

Objective: To investigate the inhibition ofSKOV3 cell by applying RNAi technique to silence signal transducers and activators of transcription 3 (STAT3)gene. Methods: shRNA templates was designed on the basis of STAT3 gene sequence and was cloned into pSilencer2.1-U6-neo vector. The resultant plasmid was transfected into SKOV3 cells with Lipofectamine 2000. The STAT3 protein and mRNA were detected by Western blot and RT-PCR,respectively. The cellular growth activity was assayed by MTT,and the apoptosis was tested by flow cytometry. Results: STAT3 protein and mRNA expression in target siRNA group were decreased ,respectively. In target siRNA group,cell proliferation was inhibited obviously and apoptosis rate increased significantly. Conclusion: Silencing STAT3 gene by the RNAi technology can decrease the expression of STAT3 gene,suppress the growth and proliferation of SKOV3 cell,and induce apoptosis of SKOV3 cell.