Changes of Glutamate,brain water content and apoptosis around hematoma and the effect of basic fibroblast growth factor on acute intracerebral hemorrhage in rats

Objective To study the effects of basic fibroblast growth factor in dynamic changes of behavior,cerebral edema,Glutamate(Glu) content and apoptosis around hematoma of acute intracerebral hemorrhage(ICH) and the relationship between them.Methods 112 Wistar rats were randomly divided into ICH group and ICH+bFGF group.The two groups were divided by time points(before ICH,4h,6h,12h,24h,72h and 7d after ICH).The rats were performed behavioral test and detected cerebral edema on 1d,2d,3d,and 7d after ICH.The change of Glu around hematoma on ICH were determined by chemical method.By terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)method,the changes of apoptosis cells around hematoma on ICH were detemined.Results Cerebral edema peaked around the third day after ICH and remained for more than a week.bFGF signifieantly improved the neurology dysfunction of rats through the decreased scores of behavioral test and brain edema was also attenuated(P0.05,P0.01).The Glu content around hematoma began to increase at 4h(P0.01),reached the top at 12h.The apoptosis around hematoma was detected at 6h and peaking at 12h(P0.01).Until 7d there had still been more apoptosis.The Glu content and apoptosis significanly decreased after bFGF interference around hematoma of ICH(P0.01).Conclusion The neurology dysfunction and brain edema were presented obviously in ICH rats,apoptosis lasted long time around hematoma on ICH.Glu content increased the number of apoptosis and bFGF decreased it.During ICH,increased release of glutamate and over stimulated glutamate receptors produce excitotoxin,which leads neuron to excessive excitement,degeneration and death.