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Optimization of Culture Medium of Lactobacillus dellbrueckii RF_(X1)

Shiling Lu

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Abstract

Response surface methodology was used to optimize the wort-based culture medium of Lactobacillus dellbrueckii RFX1.The effects of twelve additives and the initial pH of the media were evaluated by using a Plackett-Burman design.Results showed that beef extract,yeast extract,soya peptone and the initial pH of the media were the main influential factors,while the other additives had no significant effect on the cell multiplication of Lactobacillus dellbrueckii RFX1.Besides,the central composite design and response surface analysis were used to determine the optimal values of the main factors.Using the optimized culture medium,cell production of RFX1 was increased from 3.53×108 cfu/ml to 1.61×109 cfu/mL.

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What this paper is about

Response surface methodology was used to optimize the wort-based culture medium of Lactobacillus dellbrueckii RFX1.The effects of twelve additives and the initial pH of the media were evaluated by using a Plackett-Burman design.Results showed that beef extract,yeast extract,soya peptone and the initial pH of the media were the main influential factors,while the other additives had no significant effect on the cell multiplication of Lactobacillus dellbrueckii RFX1.Besides,the central composite design and response surface analysis were used to determine the optimal values of the main factors.Using the optimized culture medium,cell production of RFX1 was increased from 3.53×108 cfu/ml to 1.61×109 cfu/mL.

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Available abstract

Response surface methodology was used to optimize the wort-based culture medium of Lactobacillus dellbrueckii RFX1.The effects of twelve additives and the initial pH of the media were evaluated by using a Plackett-Burman design.Results showed that beef extract,yeast extract,soya peptone and the initial pH of the media were the main influential factors,while the other additives had no significant effect on the cell multiplication of Lactobacillus dellbrueckii RFX1.Besides,the central composite design and response surface analysis were used to determine the optimal values of the main factors.Using the optimized culture medium,cell production of RFX1 was increased from 3.53×108 cfu/ml to 1.61×109 cfu/mL.

Key concepts: Plackett–Burman design, Food science, Yeast extract, Response surface methodology, Lactobacillus, Central composite design, Chemistry, Fermentation

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