Identification of Differential Expressed Proteins Responding to Water-Stress in Seedling of Wheat Cultivar Jinmai 47

To better understand the adaptation mechanisms and differential expressed proteins of wheat to water-stress,a comparative proteome analysis was conducted in this study under normal water supply and-0.5MPa PEG-6000 solution using two-dimensional gel electrophoresis and mass spectrometry.Among 850 protein spots reproducibly detected under the two treatments,31 protein spots showed a reproducible significant change between control and stressed samples(P0.05).Of these,17 protein spots under the stress condition were up-regulated,whereas 14 protein spots were down-expressed.MALDI-TOF MS analysis successfully identified 26 of 31 differently expressed protein spots with probability based on MOWSE score in NCBI nr database,in which 19 protein spots were known functional proteins,whereas 7 protein spots were unknown.The known functional proteins identified were involved in various biological processes such as energy,metabolism,disease and defense,cell structure,transcription and translation and signal transduction.From this study,it suggested that there were several physiological metabolisms and regulatory pathways response to water-stress,and the relationship between water-stress and differential expressed proteins were also analyzed.