Optimization of technologic parameters of preparation porcine plasma protein antioxidant activity peptide by enzymatic hydrolysis

The optimum hydrolyzing conditions of preparation porcine plasma protein and antioxidant activity of porcine plasma protein hydrolysates in a liposome system were studied in this paper.Porcine plasma protein was hydrolyzed with six proteolytic enzymes(Alcalase,neutrase,flavourzyme,protamex,trypsin and papain)at their respective optimal conditions.Degree of hydrolysis was determined using pH-Stat method.Antioxidant activity(in a liposome system with thiobarbituric acid-reactive substances as the indicator),reducing power of the various hydrolysates were evaluated.The results showed the kind of protease,preheating and degree of hydrolysis of porcine plasma protein could significantly affect the antioxidant activity.Of all hydrolysates obtained by individual enzymes,the hydrolysate prepared by Alcalase had the highest antioxidative activity.The result of optimization of hydrolyzing condition of porcine plasma protein:porcine plasma protein was hydrolyzed for 5h by Alcalase,substrate concentration for 4%,and [E/S] for 2%.