Construction of eukaryotic expression vector of RNA interference specific for vascular endothelial growth factor

[Objective] To construct eukaryotic expression vector of siRNA specific for VEGF as a molecular target tool to cleave VEGF mRNA in NSCLC cell,and to expore the feasibility of it.[Methods] Genome sequences of VEGF fusion gene was retrieved from Genbank,siRNA(small interfering RNA) was designed according to the Reynolds's principle of RNAi-based medicine,and was converted into cDNA coding expression of shRNA(small hairpin RNAs) of siRNA for VEGF fusion gene.The cDNA was synthesized and inserted into plasmid pRNAi-H1.The pRNAi-H1 VEGF siRNA of recombinant plasmid being eukaryotic expression vector was controlled by the H1 promoter of RNA polymeraseIII,and identified by the restriction map,PCR and the sequence analysis.[Results] The pRNAi-H1 VEGF siRNA of recombinant plasmid identificated by the restriction map and the sequence analysis completely coincided with the designs.[Conclusion] The siRNA eukaryotic expression vector against VEGF mRNA has been successfully conctructed.