Effect of sevoflurane preconditioning on NF-κB and ICAM-1 expression in rats during liver ischemia-reperfusion injury

Objective To investigate the role of the nuclear factor-κB(NF-κB)and intercellular adhesion molecule-1(ICAM-1)in hepatic ischemia-reperfusion(IR)injury after sevoflurane preconditioning.MethodsForty female adult SD rats were randomly divided into 4 groups(n=10):Sham-operation group(N);Ischemia-reperfusion group(IR);Sevoflurane group(S);Sevoflurane ischemia-reperfusion group(SIR).The animals were killed after hepatic ischemia reperfusion injury.Serum ALT and AST were detected as liver damage markers.The liver microscopy and ultrastructure were also obtained.The levels of NF-κB and ICAM-1 in the hepatic tissues were detected by immunohistochemistry.ResultsALT and AST enzymes were significantly inhibited in SIR(P0.05).The injury score of liver ultrastructure and the levels of NF-κB and ICAM-1 were lower in SIR than those in IR(P0.05).ConclusionConclusion Sevoflurane inhibits hepatic expression of NF-κB and ICAM-1 after ischemia-reperfusion injury.NF-κB might be a regulator of ICAM-1 in this process.