Identification of Candida Species and the Hemolytic Activity Isolated from Endometritis Cattle

Candida stain is a conditional pathomycete that could be isolated from the bovine with the disease of endometritis.The 183 Candida strains were isolated from the uterus mucus of bovine with disease endometritis were used to identified at the level of generic by API20CAUX.The most commonly could be isolated were Candida krusei which accounted for 33.9%,followed by Candida rugosa were 17.5%,and the Candida kefyr,Candida albicans,Candida tropicalis were 13.1%,11.5%,8.7%.The seldom could be isolated were Candida zeylanoides,Candida parapsilosis,Candida guilliermondi,Candida fanata,Candida glabrata were 5.5%,4.4%,3.3%,1.1%,1.1%.In addition,we had also tested the active of hemolyzation in vitro of the Candida stain.Candida krusei,Candida kefyr,Candida albicans,Candida tropicalis,Candida zeylanoides and Candida glabrata display α and β haemolysis post inoculation 48 h.Candida rugosa,Candida guilliermondi,Candida fanata only display α haemolysis,Candida parapsilosis didn't display any haemolysis after incubation 72 h.Candida albicans and Candida kefyr had a higher haemolysis than others.This study lays the foundation for future study of clinical prevention and treatment of fungal endometritis and pathogenesis.