1999•Unpublished venueRequires access
Molecular pathway of G1 arrest of thymocytes induced by ionizing radiation
Guizhi Ju, MH Radiobiology
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Abstract
Objective To investigate the molecular pathway of
G1 arrest of thymocytes induced by ionizing radiation. Methods Flow cytometry (FCM) was
used to analyze cell cycle stained with propidium iodide (PI).And FCM was also used to analyze
protein expression of thymocytes for their immunofluorescence stained with monoclonal
antibodies. Results It was found that G1 phase cells of thymocytes increased significantly 12
h after whole body irradiation (WBI) with doses of 05,10 and 20 Gy,and 24 h following 2 Gy WBI
in mice.The results also showed the increases of p53+ cells of thymocytes at 1 h to 8 h,p21+
cells at 4 h to 48 h,and MDM2+ cells at 4 h and 8 h after 2 Gy WBI in mice.But no change was
found in GADD45+ cells. Conclusion Z]G1 arrest could be induced by single WBI with 05 Gy to
20 Gy Xrays,and its molecular control might be established through the p53-p21 pathway.