Screening of EST-SSRs Markers Related to Growth Traits in Crucian Carp (Carassius auratus)
Sun Xiao-wen
Abstract
Sun Xiao-wen
Abstract
Crucian carp(Carassius auratus)is rich in genetic diversity,and becomes a good genetic resource for research of molecular markers or genes associated with growth traits.A GLM procedure was used to analyze the effects of these 286 microsatellites on body length,body height,and the ratio of them.Permutation tes(t 10 000 replicates)showed that 53 makers were significant correlation with the three growth traits(P0.05),24 makers were significant correlation with the body length(P0.05),and 6 were significantly related with body length(P0.01);30 makers were significant correlation with the body height(P0.05),and 9 were significantly related with body length(P0.01);23 makers were shown to fit the correlation from the body length(P0.05),and 9 were very significant correlation(P0.01).Markers and gene types related to the three growth traits were obtained from this research,which provided effective basis to the study of crucian carp marker-assisted breeding.
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Crucian carp(Carassius auratus)is rich in genetic diversity,and becomes a good genetic resource for research of molecular markers or genes associated with growth traits.A GLM procedure was used to analyze the effects of these 286 microsatellites on body length,body height,and the ratio of them.Permutation tes(t 10 000 replicates)showed that 53 makers were significant correlation with the three growth traits(P0.05),24 makers were significant correlation with the body length(P0.05),and 6 were significantly related with body length(P0.01);30 makers were significant correlation with the body height(P0.05),and 9 were significantly related with body length(P0.01);23 makers were shown to fit the correlation from the body length(P0.05),and 9 were very significant correlation(P0.01).Markers and gene types related to the three growth traits were obtained from this research,which provided effective basis to the study of crucian carp marker-assisted breeding.
Key concepts: Crucian carp, Biology, Carassius auratus, Correlation, Microsatellite, Genetic diversity, Zoology, Genetics