The healing effects of transplanting the composite skin to the rat wounds

Objective To observe the effects of the recombinant composite skin graft consisting of epithelium,fibroblasts and acellular xeno-dermis on full thickness skin loss wounds,so as to find a new wound covering material.Methods Fifty-four SD rats with full thickness skin loss on the back were divided into three groups as A,B and C and grafted with the composite skin A(keratinocytes+ time points after the operation.Simultaneously,wound tissue samples were harvested for histological examination.Results The wound healing conditions in A group was best in the three groups,the contraction rate in A group was obviously lower than that in B group and C group(P0.05).It was indicated by histological examination that there was full differentiation of epithelium,orderly collagen proliferation and obvious reconstruction of the epithelial-dermal conjunction structure in A group.The was no obvious sign of acute immune rejection.Conclusion The wound covering with recombinant composite skin graft of epithelium,fibroblasts and acellular xeno-dermis could improve wound healing quality.The composite skin could be a new skin substitute.