Optimization of Fermentation Conditions for Antibacterial Activity Substance Produced by Streptomyces TD-1

Fermenting conditions for antibacterial activity substance produced by Streptomyces TD-1 was optimized by orthogonal and homogeneous test design method.The effects of different experimental factors on Streptomyces TD-1 producing antibiotic substance were studied.The results showed that the most important factors which influence the antibacterial activity were glucose,soy flour,FeSO4,K2HPO4 and KNO3;the optimized cultural conditions were the initial pH 7.15,culture temperature 30 ℃,6% of inoculum,the amount of shake flask 90 mL/250 mL,shaking speed 180 r/min and fermentation time 8 d.The final results showed that the antifungal activity of the fermentation broth could increase by 53.38%.