Subgingival flora analysis of chronic periodontitis patients with type-2 DM

Objective:To provide reference for prevention and treating periodontitis by comparing the subgingival flora of type-2 DM patients of chronic periodontitis with non-DM periodontitis.Method: Samples were taken from 64 diseased-teeth of 64 patients,21health-teeth of 21 normal people. Patients were taken from chronic periodontitis patients with type-2 DM and chronic periodontits patients with non-DM. A isolated cultivation of subgingival plaque samples was performed by a anaerobe non-selected media and other selective media. The P.intermedia(P.i) of the black colony in the non-selective media was detected by PCR. Result: When the sex,age,clinical marker including PLI,GI,BOP,PD,AL were not significantly different, the constituents of subgingival flora of the type-2 DM patients with chronic periodontitis were similar to those of the non-DM chronic periodontits patients; the superiority germ of type-2 DM patients was the anaerobe, specially black germs, F.n, P.i, and Capnocytophaga(P0.01);the amount of total anaerobe, total Streptococcus spp, Lactobacillus,and F.n was fewer in chronic periodontitis with type-2 DM patients(P0.05,0.05,0.01,0.01). Conclusion: The subgingival flora of type-2 DM patients with chronic periodontitis are similar to those of the non-DM chronic periodontitis patients, the amount of pathogenic germs is fewer in chronic periodontitis with type-2 DM patients.