Effects of Different Platelet-rich Plasma Preparation Methods on the Concentration of Platelet

Objective To explore an ideal method for PRP concentration by double centrifugation.Methods Totally 2.7 milliliters whole blood was collected from central ear artery of the albino rabbit,and centrifuged according to different conditions(Speed:1 000-2 000 r/min;time:5-20 min).Calculating of the platelet concentration and recovery percent of platelet were performed.Results Several centrifuge conditions successfully prepared PRP.Initial centrifugation at 1 400 r/min for 10 min,which is the critical condition for the formation of Albuginea,achieved highest rate of platelet recovery,and the operation of upper plasma is simple.Second centrifuge condition was set for 1 400 r/min 10 min.Platelet counts in PRP prepared by four different methods were increased with respect to venous blood platelet counts.Percent recovery of platelet by the second method was observed to be significantly higher when compared to other methods.Conclusion Double centrifugation is an efficient method for PRP preparation.Initial centrifugation conditions at speed of 1 400 r/min for 10 min and followed with another centrifugation at 1 400 r/min( 10 minutes) has the higher platelet count and percent recovery of platelet.It could be an efficient method for PRP preparation.