Effect of propofal on expression of Bcl-2 and P53 induced by hepatic ischemia-reperfusion in rats

Objective To investigate the effect of propofol on cell apoptosis induced by hepatic ischemia-reperfusion in rats.Methods Fifty-four SD rats were randomly divided into 3 groups(n=18):sham-operated control group(group Ⅰ),ischemia-reperfusion group(group Ⅱ)and propofol group(group Ⅲ).Each group was divided into 3 time phases at 1 h,2 h,3 h after reperfusion to establish a rat model of hepatic ischemia-reperfusion.The left hepatic lobes were sampled at the end of reperfusion.Bcl-2 and P53 protein expression in liver tissue was assessed by immunohistochemical method.The apoptosis index(AI)of hepatic cell were measured by TUNEL method.The histological change of liver were observed using light microscopy.Results Bcl-2,P53 protein expression and apoptosis index of hepatic cell were statistically different between group Ⅰ,group Ⅱ and Ⅲ(P0.05).Bcl-2 protein expression was significantly higher while P53 protein expression and AI significantly lower in group Ⅲ than those in group Ⅱ(P0.05).The pathological lesions were lighter in group Ⅲ than those in group Ⅱ under light microscope.Conclusion Propofol can relieve the liver cellular apoptosis by regulating Bcl-2 and P53 protein expression.It may protect rats from hepatic ischemia-reperfusion injury.