Cloning and Sequence Analysis of a Full-Length cDNA of β-1,3-Glucanase Gene from Musa pisangawake

【Objective】 The objective of this study is to clone β-1,3-glucanase gene which is a group of important emzymes in plant defenses against fungal diseases,and to research their resistant functions and effective applications.【Method】A novel DNA fragment encoding β-1,3-glucanase was isolated from Musa pisangawake(AA) by applying a PCR strategy in which a pair of degenerated primers deduced from the two blocks with specific conserved amino acid regions in multiple plants was used.Then the full length cDNA(glu) of the fragment was obtained by rapid amplification of cDNA ends(RACE).【Result】The full length of glu is 1 114 bp.Compared with other sequences of β-1,3-glucanase in GenBank,the deduced amino acid sequences of glu shared 54%-71% in homology.【Conclusion】 A novel β-1,3-glucanase gene was cloned from the Musa pisangawake(AA).The result reported in this paper has laid a the foundation for further studies of β-1,3-glucanase gene functions and development of transgenic plants to control plant diseases.