Comparative Analysis of Induction and Somatic Embryo genesis of Different Origin in Sorbus pohuashanesis Hedl.
Jianyin Zhang
Abstract
Jianyin Zhang
Abstract
Taking the mature and immature zygotic embryos of Sorbus pohuashanensis Hedl.as explants,the effect of areas,development time and combinations of plant growth regulators on the induction of somatic embryos and callus induction were studied.The results showed that mature zygotic embryos were used as explants,the effect of MS+2.0mg/L NAA+1.5mg/L 6-BA on callus induction and somatic embryogenesis was good.Different origin of somatic embryogenesis differences for DunhuaHarbinWuyingShanhe.In order to immature zygotic embryos as explants,MS+0.05mg/L 2,4-D+1.0mg/L 6-BA callus induction rate was high,somatic embryogenesis in MS+2,4-D0.2mg/L+1.0mg/L 6-BA was the most suitable.Different origin of somatic embryogenesis differences for YanjiHarbinYichun.
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Taking the mature and immature zygotic embryos of Sorbus pohuashanensis Hedl.as explants,the effect of areas,development time and combinations of plant growth regulators on the induction of somatic embryos and callus induction were studied.The results showed that mature zygotic embryos were used as explants,the effect of MS+2.0mg/L NAA+1.5mg/L 6-BA on callus induction and somatic embryogenesis was good.Different origin of somatic embryogenesis differences for DunhuaHarbinWuyingShanhe.In order to immature zygotic embryos as explants,MS+0.05mg/L 2,4-D+1.0mg/L 6-BA callus induction rate was high,somatic embryogenesis in MS+2,4-D0.2mg/L+1.0mg/L 6-BA was the most suitable.Different origin of somatic embryogenesis differences for YanjiHarbinYichun.
Key concepts: Somatic embryogenesis, Callus, Explant culture, Embryo, Somatic cell, Zygote, Biology, Embryogenesis