2007•Unpublished venueRequires access

Influencing Factors on the Efficiency of Gene Transformation System of the Monascus purpureus 9903A

Yong Xia

Abstract

The protoplasts from Monascus purpureus 9903A were transformed with hygromycin B resistance plasmid pMP-Hygro.The transformation medium contained 50 mmol/L Ca 2+ and 40%PEG4000 with 1.0mol/L sorbitol an osmotic stabilizer.The protoplasts concentration is1×10 8 /mL and the quantity of vectors is 1 μg/100μL protoplasts.In this study,the average transformation ratio is 160 colonies/μg DNA.

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Key concepts: Monascus purpureus, Protoplast, Sorbitol, Transformation (genetics), Hygromycin B, Plasmid, Food science, Biology