Influence of TRPV2 silencing on prostate cancer cell PC-3 proliferation and migration

Objective To explore the effects of TRPV2 gene silencing by small interfering RNA(siRNA)on the proliferatoin,cell cycle and migration of prostate cancer cell line PC-3.MethodssiRNA targeting TRPV2 gene was constructed and transfected into PC-3 cells mediated by liposome.Expression of TRPV2 mRNA was assayed by RT-PCR.The proliferation was detected by MTT method.The cell cycle was determined by FCM.The migration was detected by Transwell test.ResultsTRPV2 mRNA was expressed in androgen-independent PC-3 cells and DU-145,which was not expressed in androgen-dependent prostate cancer cell line LNCaP.Expression of TRPV2 mRNA in PC-3 cells was decreased in the extension of siRNA transfection.At 72h after transfection,the expression of TRPV2 mRNA was decreased by 85% compared with the blank and negative control group(P0.01).The number of migration cell in siRNA transfection group was significantly less than that in blank group or negtive control group[(46.0±6.7)vs.(92.0±9.4)or(109.0±8.1)](P0.01).Conclusion TRPV2 is expressed in androgen-independent PC-3 cells and DU145 cells,which can be effectively inhibited by siRNA targeting TRPV2 gene.siRNA targeting TRPV2 gene has no effect on the cell proliferation and cell circle.