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Production and Identification of Polyclonal Antibody against Triazophos

Yuanming Sun

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Abstract

In this paper,artificial antigens for triazophos including one immunogen and one coating antigen were prepared.The hapten(TZPM-Hap) of triazophos was coupled to bovine serum albumin(BSA) and ovalbumin(OVA) by the active ester method to prepare the artificial immunogen TZPM-A-BSA and the coating antigen TZPM-A-OVA,respectively.Immunogen and coating antigen were successfully prepared and confirmed by ultraviolet spectrometry.Polyclonal antibodies against triazophos were produced from female Balb/c mice,which were immunized with the artificial immunogen TZPM-A-BSA.Indirectly competitive ELISA was established with this antiserum,of which IC50 was 0.01 μg/mL.The polyclonal antibodies showed no obvious cross-reactivity with other structural-related organophosphate pesticides.

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What this paper is about

In this paper,artificial antigens for triazophos including one immunogen and one coating antigen were prepared.The hapten(TZPM-Hap) of triazophos was coupled to bovine serum albumin(BSA) and ovalbumin(OVA) by the active ester method to prepare the artificial immunogen TZPM-A-BSA and the coating antigen TZPM-A-OVA,respectively.Immunogen and coating antigen were successfully prepared and confirmed by ultraviolet spectrometry.Polyclonal antibodies against triazophos were produced from female Balb/c mice,which were immunized with the artificial immunogen TZPM-A-BSA.Indirectly competitive ELISA was established with this antiserum,of which IC50 was 0.01 μg/mL.The polyclonal antibodies showed no obvious cross-reactivity with other structural-related organophosphate pesticides.

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Available abstract

In this paper,artificial antigens for triazophos including one immunogen and one coating antigen were prepared.The hapten(TZPM-Hap) of triazophos was coupled to bovine serum albumin(BSA) and ovalbumin(OVA) by the active ester method to prepare the artificial immunogen TZPM-A-BSA and the coating antigen TZPM-A-OVA,respectively.Immunogen and coating antigen were successfully prepared and confirmed by ultraviolet spectrometry.Polyclonal antibodies against triazophos were produced from female Balb/c mice,which were immunized with the artificial immunogen TZPM-A-BSA.Indirectly competitive ELISA was established with this antiserum,of which IC50 was 0.01 μg/mL.The polyclonal antibodies showed no obvious cross-reactivity with other structural-related organophosphate pesticides.

Key concepts: Immunogen, Polyclonal antibodies, Hapten, Antiserum, Antigen, Bovine serum albumin, Ovalbumin, Chemistry

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Production and Identification of Polyclonal Antibody against Triazophos — Research Paper | ScholarLens