The Effect of Injection of Terbutaline Early on Rats with Acute Lung Injury

Objective To observe the effect of intravenous injection of terbutaline early on the rats with acute lung injury.Methods Forty SD rats were randomly assigned into 4 groups:NS control group(group A),ALI group(group B),ALI+terbutaline treatment group(group C) and ALI+terbutaline+ouabain intervention group (group D) with 10 in each group.The rats of all groups were respectively anesthetized by intraperitoneal injection of 10% chloral hydrate (3.5 μL·g-1).After being anaesthetized successfully,the rats in group A were injected with 5 mg·kg-1 NS through the tails vein within 1 hour;the rats in group B were injected with 5 mg·kg-1 LPS (which dissolved in 1 mL of NS) throgh the tails vein in 4 times within 1 hour;the rats in group C and D were injected with 5 mg·kg-1 LPS (which dissolved in 1 mL of NS) through the tails vein in 4 times within 1 hour.Then the rats were injected with 5.0 μL·g-1 terbutaline within 1 hour.Then elevated the rats head to 45 degrees,the tracheal intubation was performed under lights illuminating the rats neck,after intubated successfully,1 mL·kg-1 Ringer’s solution was dripped into trachea in groups A,B and C,and D with 1 mL·kg-1 Ringer’s solution which contain 10-3 mol·L-1 ouabain.To establish Lipopolysaccharide-induced model of acute lung injury in rats,the pathomorphologic scores of lung injury,the wet to dry weight ratio (W/D),and the activity of Na+-K+-ATPase were determined after 6 hours,and bronchoalveolar lavage fluid (BALF) cells count,the percentage neutrophilicgranulocyte (PMN),BALF concentration of protein were determined too.Results The pathomorphologic scores of lung injury,the wet to W/D,BALF cells count,the PMN,BALF concentration of protein in group A were significantly lower than that in group B,C,and D(all P0.05),the above mentioned parameters in group B and D were higher than those in group C(all P0.05),but there were no significant differences between groups B and D(P0.05).the activity of Na+-K+-ATP ase in group A were significantly lower than that in groups B,C,D(P0.05),the activity of Na+-K+-ATP ase in groups B and D were higher than those in group C(all P0.05),but there were no significant differences between group B and D (P0.05).Conclusion The intravenous injection of terbutaline early can promote alveolar epithelial fluid reabsorption in rats with ALI via the upregulation of the activity of Na+-K+-ATP ase in lung,and the effect could control the further development of ALI.