Detection of the Th1/Th2、Tc1/Tc2 Subsets in Rheumatoid Arthritis Patients and Its Significance

Objective To investigate the polarization of T cells subset and analyze the immunologic pathogenesis in RA.Methods The ratios of CD4~+/CD8~+,CD3~+CD4~+CD30~-/CD3~+CD4~+CD30~+ and CD3~+CD8~+CD30~-/CD3~+CD8~+CD30~+ in peripheral blood T cells were analyzed by immunofluorescence staining and tris-color flow cytometry in vitro.Results Compared with the ratios of CD4~+/CD8~+(1.57±0.04) in healthy people,the ratios of CD4~+/CD8~+(1.95±0.05) in RA in creased obviously (P0.05).Compared with the ratios of CD3~+CD4~+CD30~-/CD3~+CD4~+CD30~+(15.74±5.36) and CD3~+CD8~+CD30~-/CD3~+CD8~+CD30~+(15.82±4.03) inhealthy people,theratiosofCD3~+CD4~+CD30~-/CD3~+CD4~+CD30~+(20.72±7.34)and CD3~+CD8~+CD30~-/CD3~+CD8~+CD30~+ (19.81±3.28).FCM analysis showed that average percentages of CD4~+(47.42%±2.68%) in RA increased sharply (P0.01) versus 40.27%±2.53% in healthy people,while the average percentages of CD8~+,CD3~+CD4~+CD30~+,CD3~+CD8~+CD30~- and CD3~+CD8~+CD30~+ did not changed versus healthy people.Conclusion The ratios of CD4~+/CD8~+,CD3~+CD4~+CD30~-/CD3~+CD4~+CD30~+ and CD3~+CD8~+CD30~-/CD3~+CD8~+CD30~+ in peripheral blood T cells were increased obviously in RA.It suggested that cellular immunity in RA is shifted to Th1 type immunity.