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2004•Chinese Journal of Clinical NeurosciencesRequires access

Mechanism of PC12 Cells Injury Induced by Aβ and Neuroprotective Effect of Donepezil

Li Li

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Abstract

Aim:To study the possible mechanism of cell death induced by soluble Aβ 25-35 and the protective effect of Donepezil on them.Methods:By using cell culture,MTT assays,TUNEL,electron microscope,we measured lactate dehydrogenase(LDH) release rate,cell viability,for observing the effect of Donepezil on Aβ induced apoptosis and necrosis in PC12 cells.Results:MTT assays showed that cell viability decreased under Aβ 25-35 treatment.TUNEL showed that after Aβ treatment the apoptotic cell rate was 32%.Electron microscope showed apoptosis and necrosis occurred.While under Donepezil pretreatement,the apoptotic cell rate was 17% (P0.01) and LDH release rate decreased compared with the former group.Conclusion:Aβ 25-35 induced PC12 cell apoptosis and necrosis while Donepezil could protect PC12 cells against them both.

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What this paper is about

Aim:To study the possible mechanism of cell death induced by soluble Aβ 25-35 and the protective effect of Donepezil on them.Methods:By using cell culture,MTT assays,TUNEL,electron microscope,we measured lactate dehydrogenase(LDH) release rate,cell viability,for observing the effect of Donepezil on Aβ induced apoptosis and necrosis in PC12 cells.Results:MTT assays showed that cell viability decreased under Aβ 25-35 treatment.TUNEL showed that after Aβ treatment the apoptotic cell rate was 32%.Electron microscope showed apoptosis and necrosis occurred.While under Donepezil pretreatement,the apoptotic cell rate was 17% (P0.01) and LDH release rate decreased compared with the former group.Conclusion:Aβ 25-35 induced PC12 cell apoptosis and necrosis while Donepezil could protect PC12 cells against them both.

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Available abstract

Aim:To study the possible mechanism of cell death induced by soluble Aβ 25-35 and the protective effect of Donepezil on them.Methods:By using cell culture,MTT assays,TUNEL,electron microscope,we measured lactate dehydrogenase(LDH) release rate,cell viability,for observing the effect of Donepezil on Aβ induced apoptosis and necrosis in PC12 cells.Results:MTT assays showed that cell viability decreased under Aβ 25-35 treatment.TUNEL showed that after Aβ treatment the apoptotic cell rate was 32%.Electron microscope showed apoptosis and necrosis occurred.While under Donepezil pretreatement,the apoptotic cell rate was 17% (P0.01) and LDH release rate decreased compared with the former group.Conclusion:Aβ 25-35 induced PC12 cell apoptosis and necrosis while Donepezil could protect PC12 cells against them both.

Key concepts: TUNEL assay, Donepezil, Apoptosis, Viability assay, Lactate dehydrogenase, MTT assay, Necrosis, Neuroprotection

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