Kinds and Amount Variation of Organic Acids During Microbial Glycerol Production with C.glycerolgenesis

The kinds and amount of organic acids during microbial glycerol production with C. gly cerolgenesis were determined by HPLC.The organic acids were mainly produced in growth phase in C.glycerolgenesis which increased the total acidity of fermentation medium. There were acetic acid, lactate, pyrurate, malic acid, α ketoglutaric determined by HPLC during fermentation process. It was found that acetic acid was the highest, about 1.6 g/L. After the growth phase the total acidity of fermentation medium decreased. However, the amount of acetic acid and α ketoglutaric acid increased slightly.