Study on optimizing extraction and antioxidative activity of total flavonoids from Persimmon Leaves

Objective To study the optimum conditions of the total flavonoids from Persimmon Leaves and their antioxidant activity.Methods Adopt to single factor experiments and orthogonal test,It Was the extraction rate of total flavonoids from Persimmon Leaves was taken as evaluation index to select the optimum extraction process.The antioxidant activity of the total flavonoids from Persimmon Leaves was evaluated by DPPH radical scavenging ability in vitro.Results The optimum extraction prcess for total flavonoids from persimmon leaves was as follows:ethanol concentration was 70%,the ratio of solid to liquid was 1∶ 10,extraction time was 1 h,and extraction times was 2.The extraction ratio was 0.68% in this condition.the total flavonoids from Persimmon Leaves has showed stronger antioxidant activity,its clearance of the extract towards DPPH radical in vitro was 80.23%.Conclusion The total flavonoids from Persimmon Leaves is a safe and effective antioxidant,and its optimum extraction process is feasible for industrial production.