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2012•Acta Horticulturae SinicaRequires access

Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource

Tingting Zhao, Song NingNing, Jiang Jingbin, Zhang He, Kang LiGong, Lu JingFu, Xiangyang Xu

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Abstract

The study identified the molecular marker linked to Cladosporium fulvum resistant gene Cf12 in tomato. The linked marker was screened with a F2 population between a resistant and a susceptible parent(05HN36 × 051355)by AFLP technology. Six AFLP markers had been identified to linked with Cf12 gene:E86M51-C,E46M50,E95M59-D,E35M81,E78M36-C and E47M50-C. The genetic distances were 5.1,8.9,9.1,9.2,10.2 and 10.8 cM respectively. The AFLP marker E86M51-C was converted into SCAR marker. We obtained 16 cultivars which contained this marker. These cultivars could be used in C. fulvum resistant breeding practice.

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What this paper is about

The study identified the molecular marker linked to Cladosporium fulvum resistant gene Cf12 in tomato. The linked marker was screened with a F2 population between a resistant and a susceptible parent(05HN36 × 051355)by AFLP technology. Six AFLP markers had been identified to linked with Cf12 gene:E86M51-C,E46M50,E95M59-D,E35M81,E78M36-C and E47M50-C. The genetic distances were 5.1,8.9,9.1,9.2,10.2 and 10.8 cM respectively. The AFLP marker E86M51-C was converted into SCAR marker. We obtained 16 cultivars which contained this marker. These cultivars could be used in C. fulvum resistant breeding practice.

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Available abstract

The study identified the molecular marker linked to Cladosporium fulvum resistant gene Cf12 in tomato. The linked marker was screened with a F2 population between a resistant and a susceptible parent(05HN36 × 051355)by AFLP technology. Six AFLP markers had been identified to linked with Cf12 gene:E86M51-C,E46M50,E95M59-D,E35M81,E78M36-C and E47M50-C. The genetic distances were 5.1,8.9,9.1,9.2,10.2 and 10.8 cM respectively. The AFLP marker E86M51-C was converted into SCAR marker. We obtained 16 cultivars which contained this marker. These cultivars could be used in C. fulvum resistant breeding practice.

Key concepts: Cladosporium, Amplified fragment length polymorphism, Germplasm, Biology, Molecular marker, Genetic marker, Population, Gene

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Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource — Research Paper | ScholarLens