Effect of 1, 25 (OH)_2D_3 on interstitial fibrosis in UUO rats

Objective : To investigate the effects of 1, 25 (OH)2D3 on renal interstitial fibrosis in rat. Methods: Male SD rats (7weeks old) were divided into 4 groups at random: ①UUO group(6 rats), ②the treated group (6 rats): UUO + 1, 25(OH)2D3 per 100 g3 ng/day by intraperitoneally (I.P), ③the control group(6 rats): UUO + ethanol by I.P, ④sham operated group(6 rats). Kidneys wereharvested on day 7 after UUO. Fibrosis was assessed by measuring tissue hydroxyproline content. Tissue NF-κB expression wasdetermined by RT- PCR. Results: UUO for 7 days significantly activated NF-κB, induced interstitial fibrosis in obstructed kidney ofthe control group and UUO group compared with sham operated group rats. Conversely daily administration of 1, 25 (OH)2D3significantly inhibited NF-B expressionand decreased interstitial fibrosis in obstructed kidney of treated group. Conclusion: 1, 25(OH)2D3 might have had beneficial effects on interstitial fibrosis in SD rats with UUO, and this effect may be associated with 1, 25(OH)2D3 inhibits NF-KB expression.