The Effect of Total Spaonins Panax Notoginseng in Thrombin-induced VSMCs Proliferation and pp60c-src Activity

Objective To study the effect of total spaonins panax notoginseng (tPNS)in thrombin-induced vascular smooth muscle cells (VSMCs) proliferation and pp60c-src activity.Method Growth-arrested VSMCs (4-10 generation) were used in experiment.Cell proliferation was quantified by MTS method.The activity of pp60 c-src in cells was investigated by immunoprecipitation.Results ①Either thrombin group (0.5 or 1.0 U/mL) presents similar cell proliferation curves with two peaks,occurring at 1 h(P0.05) and at 24 h(P0.05).②tPNS at various concentrations(300.0～600.0 μg/mL) significantly decreased thrombin-induced VSMCs proliferation at 24 h(P0.05),and the effects of various concentrations of tPNS (300.0～600.0 μg/mL) were different to each other (P0.05).③Compared with thrombin group,tPNS(450.0 μg/mL)decreased the thrombin-induced cells proliferations at 1 h(P0.05) and at 24 h(P0.05). ④Compared with 0 h,the activity of pp60c-src in thrombin group increased significantly at 1 h(P0.05)and at 6、12、24、48、72 h respectively.Compared with thrombin group,tPNS decreased the the activity of pp60c-src at 1 h(P0.01)and at 6、12、24、48、72 h respectively.Conclusions ①The cell proliferation induced by thrombin has two peaks at 1 h and 24 h. ②tPNS at various concentrations significantly decreased thrombin-induced VSMCs proliferation.③The inhibition effect of tPNS in thrombin-induced VSMCs proliferation was not only quickly and transient but also torpid and late.④tPNS can inhibit the proliferation of VSMCs induced by thrombin significantly,at least in part by decreaseing the pp60c-src activity.