2002•Acta Academiae Medicinae JiangxiRequires access
Detection of Lymph Node Micrometastases in Gastric Carcinoma by Immunohistochemical and RT-PCR Methods
Wei Xiao
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Abstract
Objective: To detect lymph node micrometastases, in patients with histologically node negative gastric carcinoma and explore the clinical significance of lymph node micrometastases in gastric carcinoma.Methods:A total of 205 lymph nodes from 11 patients,with histologically node negative gastric carcinoma, were detected by immunostaining with monoclonal antibody CEA and CEA mRNA RT PCR for micrometastases respectively.Results:18 lymph nodes (8.8%) were found to have micrometastases by immunostaining with CEA antibody and 46 (22.4%) by CEA mRNA RT PCR.The two rates were significantly different( P 0.01).The TNM classification of 6 patients with lymph node micrometastases was upstaged. In the follow up, two cases with lymph node micrometastases recurred in 6 months and nine months after radical resection respectively.Conclusion:Lymph node micrometastases can be detected by immunostaining with CEA antibody or CEA mRNA RT PCR. The RT PCR assay was more sensitive.The detection of lymph node micrometastases has an important significance in determining the clinical staging and prognosis and can a direct clinical therapy of gastric carcinoma.