Peripheral blood mononuclear cells in patients with breast cancer induced by dendritic cells in vitro Treatment

Objective The peripheral blood mononuelear cells from breast cancer patients induced into dendritic cells in vitro.Methods After stem cells mobilization the peripheral blood mononuelear cells(PBMC) of patients with breast cancer were collected by cell detachment machine,Abstracted by Ficoll,suspended by RPMI-1640 nutritive medium and standing for 30 minute,then the adherent cells were cultured in RPMI-1640 nutritive medium containing 10 percent FBS,induced by adding GM-CSF 1000U/ml,IL-4 500U/ml,the 4th day 2/3 volume of exchange fluid,fill the GM-CSF,IL-4,and join the induction of TNF-α 500 U/ml mature,and the 7th day 2/3 volume of exchange fluid,making up for cytokine.Results On the 9th day we collected 1-2×105 dendritic cells and detected CD83+(47.8±6.2)%,CD86+(83.2±10.1),HLA-DR(75.9±7.9)%,CD1a(60.3±6.8)%.Conclusion After stem cells mobilization the PBMC from breast cancer patients can be induced into dendritic cells by adding GM-CSF,IL-4 and TNF-α,and will provide the basis of clinical application for cell immunotherapy of breast cancer patients.