The remodeling evaluation of osteopsathyrosis congenita adopting osteoclast-calvaria co-culture system in vitro

Objective To evaluate the effects of osteoblast(OB) and osteoclast(OC) on the bone remodeling of osteogenesis imperfecta(OI) adopting osteoclast-calvaria co-culture system in vitro on oim/oim(OI) mouse model.Methods Wild(WT) and OI mice were used and compared in this study.OC cells were cultured in calvaria(CAL) in vitro for WT(WTCAL-WTOC group) and OI mice(OICAL-OIOC group) respectively.Tartrate-resistant acid phosphatase(TRAP) staining and alkaline phosphatase(ALP) staining were used to identify OCs and OBs respectively.Bone resorption of OCs was assessed by the area percentage of absorption lacunam,which is the rate of OCs number to the whole calvarial surfaces.Results At the culturing time of d7,the number of OCs and OC/OB rate of group OICAL-OIOC were significantly lower than that of group WTCAL-WTOC.However,the OCs number normalized to resorption pit number was significantly greater in group OICAl-OIOC compared to that of WTCAl-WTOC group.Conclusions The mechanism of the increased OCs function is partially due to the increased bone turnover in OI mice model for the compensation of decreased OBs function.