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Cloning and Sequence Analysis of Partial Expansin Gene in Papaya Fruit

Scuta Haikou

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Abstract

Expansin is a group of cell-wall-localized proteins which induce expansion of cell wall. By using PCR method, partial sequences of α-expansin genes were amplified from cDNA of papaya fruit. The sequence of 531 bp encodes a polypeptide of 177 amino acids, named Cp-EXP1. The success of cloning expansin gene from papaya builds a great base for the study of the development and growth and varietal improvement of papaya.

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What this paper is about

Expansin is a group of cell-wall-localized proteins which induce expansion of cell wall. By using PCR method, partial sequences of α-expansin genes were amplified from cDNA of papaya fruit. The sequence of 531 bp encodes a polypeptide of 177 amino acids, named Cp-EXP1. The success of cloning expansin gene from papaya builds a great base for the study of the development and growth and varietal improvement of papaya.

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Available abstract

Expansin is a group of cell-wall-localized proteins which induce expansion of cell wall. By using PCR method, partial sequences of α-expansin genes were amplified from cDNA of papaya fruit. The sequence of 531 bp encodes a polypeptide of 177 amino acids, named Cp-EXP1. The success of cloning expansin gene from papaya builds a great base for the study of the development and growth and varietal improvement of papaya.

Key concepts: Expansin, Cloning (programming), Gene, Biology, Sequence analysis, Complementary DNA, Sequence (biology), Peptide sequence

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