Study of the Relationship between Angiogenic Factors and Mechanism of Preeclampsia

Objective:To study the relationship between serum soluble intercellular adhesion molecule-1(sICAM-1) and vascular endothelial growth factor(VEGF), placenta growth factor(PLGF), vascular endothelial growth factor receptor 1(VEG- FR1, Flt-1), soluble vascular endothelial growth factor receptor 1 (sVEGFR1, sFlt-1) mRNA expression in placenta tissue of preeclampsia (PE). Methods: The serum level of sICAM-1 was measured by enzyme-linked immunosorbent assay and gene expression of placenta tissue was detected by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Re- sults:(1)The average serum level of sICAM-1 was(218.45±29.93) μg/L in PE group compared with (168.84±19.39) μg/L in controls (P 0.01).(2)The mRNA expressions of VEGF, PLGF, Flt-1 and sFlt-1 were increased in placenta of PE than those in controls (P 0.01).(3)There was a positive correlation between the serum level of sICAM-1 and the sFlt-1 mRNA expres- sion in placenta tissue (r = 0.90, P 0.01). Conclusion: The serum level of sICAM was increased in the patients with PE. The expressions of VEGF, PLGF, Flt-1 and sFlt-1mRNA were increased remarkably in the placenta tissue of palients with PE,es- pecial for sFlt-1. The remarkable increase for expression of sFlt-1mRNA may contribute to endothelial dysfunction, hyperten- sion and proteinuria.