Effect of peroxisome proliferator activated receptor δ on mRNA expression of MCP-1 induced by homocysteine in cultured human umbilical vein endothelial cells

AIM: To investigate the effects of peroxisome proliferator activated receptor δ(PPARδ) on the mRNA expression of monocyte chemoattractant protein 1(MCP-1) induced by homocysteine(Hcy) in human umbilical vein endothelial cells(HUVECs).METHODS: Collagenase was used to isolate endothelial cells from human umbilical vein,and the cells were cultured in vitro.The HUVECs were divided into blank control group,Hcy group,GW0742(a specific agonist of PPARδ) group and diphenyleneiodonium(DPI,a specific inhibitor of NADPH oxidase) group.RT-PCR was used to examine the mRNA expression of MCP-1 and PPARδ.The protein level of PPARδ was detected by Western blotting.2',7'-Dichlorofluorescin diacetate(DCFH-DA) was added to monitor intracellular production of reactive oxygen species(ROS).RESULTS: Compared with control group,Hcy promoted the mRNA expression of MCP-1 in a concentration-dependent manner,and decreased the mRNA expression of PPARδ in HUVECs.The mRNA expression of MCP-1 was significantly elevated by Hcy at the concentration of 10-5 mol/L,and the mRNA expression of PPARδ was decreased remarkably(P0.01).GW0742 decreased the mRNA expression of MCP-1 compared with Hcy group(P0.01).Hcy remarkably increased the production of ROS compared with control group.Hcy-induced production of ROS was also significantly attenuated by GW0742.CONCLUSION: The activation of PPARδ decreases the Hcy-induced mRNA expression of MCP-1 by suppressing Hcy-stimulated production of ROS.