A retrospective analysis of antinuclear antibody test of 752 specimens

Retrospective analysis on the results of antinuclear antibody test,to investigate the correlation between indirect immunofluorescence assay for screening ANA and line immunoassay for specific ANA,752 specimens were tested by IIF-ANA and LIA-ANAs.Among 752 cases,the positive rate of the two methods were 27.26%(IIF) and 27.66%(LIA),and two methods showed no statistically difference(P=0.875,kappa=0.463).ANA-positive rate of men and women showed significant difference(15.5% vs.32.71%,P=0.000).The detection rate of IIF for specific autoantibodies such as anti-Jo-1,anti-PM-Scl and anti-Scl-70 is low.IIF+/LIA+ patients had high-titer≥ 1∶1000,accounting for 64.3%,while IIF+/LIA-patients had low-titer 1∶100,accounting for 50.5%.IIF can be used for screening the antinuclear antibodies,then LIA for confirmation.For those IIF+/LIA-patients: if they have obvious clinical symptoms of AID but IIF test is negative,LIA test should be performed for confirmation;for those IIF+/LIA-patients with high titer,especially in those patients with subclinical symptoms,clinical attention should be given,and regular follow-up is recommended.