The effects of iron on apoptosis of HL-60 cells induced by Ara-C

Objective: To explore the effects of iron on apoptosis of HL 60 cells induced by Ara C.Methods: HL 60 cells were incubated at 37 ℃ in RPMI 1640 containing 10% heat-inactived fetal bovine serum in an water-saturated 5% CO 2 incubator.apoptosis of HL 60 cells was investigated by cell morphology,flow cytometry assay,DNA gel electrophoresis.Through the experiment,chosing 100 μmol/L FeCl 3?10 μmol/L DFO and Ara C(1 μg/ml?10 μg/ml?100 μg/ml)as the experimental concentration.Results: ①When HL 60 cells cultured with 100 μmol/L FeCl 3+ 100 μg/ml Ara C 6h,12h 24h,APO% were higher than that of Ara C;and sub G1 declined remarkably in comparison with Ara C group ( P 0.01).DNA electrophoresis showed that ladder numbers decreased and the present time of ladder lagged.②Compared 10 μmol/L DFO+ 100 μg/ml Ara C with Ara C,APO% of former was much higher than that of the latter ( P 0.05),Sub G1% of DFO+Ara C group was higher than that of Ara C.③Equimolar concentration of FeCl 3 and DFO+ Ara C,the APO% and Sub G1% was no significant difference with Ara C.Conclusion: Iron overload could inhibited apoptosis of HL 60 cells induced by Ara C,Iron deprivation may promote apoptosis of HL 60 cells induced by Ara C.So in clinical,the iron deprivation could have a place in the treatment of leukemia in conjuncting with other anticancer agents and supplement of iron to leukemic patients shoud be careful.