Construction and expression of TAT-EGFP fusion protein expression vector

Aim: To construct an expression vector of TAT-EGFP and express the fusion protein in E.coli BL21.Methods:A synthesized DNA fragment encoding TAT protein transduction domain was inserted into pET28a vector, then EGFP gene was ligated to construct the expression vector of pET28a-TAT-EGFP. The recombinant vector was transformed into E.coli BL21 and induced with IPTG. The highly expressed TAT-EGFP was purified by affinity chromatography.Results and Conclusion: pET28a-TAT-EGFP is successfully constructed and fusion protein TAT-EGFP is successfully expressed in prokaryotic cells, which lays the foundation for studying the protein transduction of TAT.