Effect of darbufelone on the proliferation and apoptosis of SGC-7901 cell line

Aim To investigate the effects of dual COX-2/5-LOX inhibitor darbufelone on the inhibition of gastric adenocarcinoma in vitro.Methods MTT assay was used to detect the inhibition of gastric adenocarcinoma cell line SGC-7901 by darbufelone in different concentrations and at different times.TUNEL staining was used to evaluate the apoptosis of SGC-7901 cells.The changes of 5-LOX and COX-2 mRNA expression were studied by reverse transcription polymerase chain reaction.5-LOX and COX-2 protein expressions were analyzed by immunocytochemistry in SGC-7901 cells.Results darbufelone could decrease the proliferation significantly in a dose-dependent and time-dependent manner in SGC-7901 cells.After treating SGC-7901 with darbufelone at concentration of 1.5×10-5,1.0×10-5,5×10-6 mol·L-1 for 72 hs,apoptosis index(%) of SGC-7901 was(30.3±2.1)%,(23.0±2.0)%,(15.0±1.5)%,respectively,which was significantly higher than(0.6±0.1)% that in control group(P0.01).1.5×10-5,1.0×10-5,5×10-6 mol·L-1 darbufelone effectively inhibited the protein and mRNA expression of COX-2 and 5-LOX(P0.05).Conclusions darbufelone can inhibit the proliferation and induce apoptosis of SGC-7901 cells.