Study on the induction of apoptosis in BHK-21 cells by FMDV

In this study,the apoptosis of BHK-21 cells induced by Foot-and-mouth disease virus(FMDV)was reported.The methods of MTT,fluorescence staining of Hoechst 33258,TdT-mediated dUTP nick-end-labeling(TUNEL),flow cytometry and strand-specific real-time RT-PCR were used to assay the inhibitory effects of FMDV on the growth of BHK-21 cells,the morphological features of apoptotic cells,the apoptosis apex,the cell cycle and the RNA product of replication system in cells respectively.The results showed that FMDV could inhibit the growth of BHK-21 cells,induce apoptosis apex and the cell cycle was held back at G1/G0 phase,in addition,the apoptosis rate increased in dose-dependent as well as time-dependent manners and presented some relationship with the RNA product of replication system in BHK-21 cells.This work founded a platform for the mechanism study of apoptosis induced by FMDV.