Study on experimental conditions for phagocyteosis of mouse peritoneal macrophages

Objective To provide a reliable,sensitive and stable method for detecting the percentage of phagocytosis of mouse peritoneal macrophages.Methods The mouse peritoneal macrophages were incubated with latex beads in 24 well plate in cell culture incubator.The percentage of phagocytosis(PP) and phagocytosis index(PI) were detected by fluorescence microscope.Results As the culture time increasing,PP of macrophages became higher (P0.05).The PP of peritoneal macrophages increased with the increment of calf serum concentration.As the concentration of latex beads increasing,the PP of peritoneal macrophages also increased(P0.01).The PP of peritoneal macrophages of adherent macrophages was higher than that of non-adherent macrophages(P0.01).The PP of peritoneal macrophages of the Balb/c mice was significantly higher than that of the C57,KM and ICR mice (P0.01).Conclusion The optimal experimental conditions of the phagocyteosis of mouse peritoneal macrophages might be the concentration of latex beads of 1‰,the calf serum concentration of 2.5%-5%,adding the latex beads and continuous culturing for 2 hours at the time of 2 hours after the adherence of macrophages.Besides,it is indicated that Balb/c mouse is most suitable for detection of the capacity of phagocyteosis.