Cytogenentics Analysis of 176 Primary and Secondary Amenorrhea Patients

Objective:To study the pathological causes of the patients with primary and secondary amenorrhea on a cytogenetics level.Methods:176 patients were selected in the study,156 primary amenorrhea and 20 secondary amenorrhea.Peripheral blood lyphocytes chromosome samples of each patient were collected,according to routine method,after the procedures of incubation,preparation,baking,pancreatin digestion and Giemsa staining.For each case,3～5 karyotypes out of 20 observed cells were analysized under microscope.When mosaic karyotypes were observed,100 karyotypes were observed.Results:48 abnormal chromosome karyotypes were detected in 176 cases,the abnormality detection rate was 27.27%,and it was mainly involved in the numerical abnormality of chromosome X and mosaic of 45,XO,including 14 patients with 45,XO,19 patients with mosaic of 45,XO,5 patients with structural abnormalities of X,6 patients karyotypes were 46,XY.There were 46 patients with abnormal karyotypes among cases with primary amenorrhea,and 2 patients with abnormal sex chromosome among cases with secondary amenorrhea.Conclusions:Chromosome abnormality is an important cause for primary and secondary amenorrhea.For those patients,the chromosome examination is necessary for the therapy and determination of amenorrhea pathogeny.