Effect of Ku70 gene interfering RNA on radiation sensitivity of cervical cancer Hela cells

Objective To observe the ku70 gene short interfering RNA(siRNA) on cervical cancer Hela cells,radiation sensitivity of.Methods Determination of colony-forming technology,different doses of-ray irradiation effect on the Hela cell viability was observed Hela cells to-ray radiation sensitivity.When using quantitative-PCR(quantitiv real time PCR,QRT-PCR) detection of expression of Ku70 mRNA level.Western-blot technique using Western Blot Detection of Ku70 protein expression.Using RNA interference(RNAi) techniques to suppress the Hela cells,Ku70 protein expression was observed decreased expression of Ku70 protein in Hela cells on radiation sensitivity.Colony formation was measured by using 4.0 Gy-ray irradiation effect on the survival rate of Hela cells.Further analysis of Hela cells,the radiosensitivity of -rays.Results The cervical cancer Hela cells to 1.0～4.0 Gy-rays is not sensitive enough,there is a very high radiation resistance.Dose-response experiments confirmed that,1.0～6.0 Gy-ray irradiation,HeLa cells and the Ku70 protein expression of Ku70 mRNA were significantly increased.Aging experiments confirmed that,4.0Gy-ray irradiation 8 h～72 h,HeLa cells and the Ku70 protein expression of Ku70 mRNA were significantly increased.In this study,the successful construction of the Ku70 gene RNA interference vector pSilencer-4.1-Ku70,stably transfected HeLa cells,effectively inhibit the expression of Ku70 protein.Stable transfection of pSilencer-4.1-Ku70 interfere with and inhibit the Ku70 protein expression plasmid,after,HeLa cells rays of radiation sensitivity was significantly higher(P 0.001).Conclusion Ku70 siRNA effectively reduced the level of Ku70 mRNA,inhibit the expression of Ku70 protein can significantly enhance the Hela cells,the degree of sensitivity to radiation.The results of this study also suggests,Ku70 gene can be used as indicators to measure radiation sensitivity of cervical cancer,screening patients for clinical radiotherapy,forecasting to provide a suitable means of radiotherapy.