Screening and identification of lactic acidbacterium producing bacteriocin with broad spectrum

One strain was screened from thirty six lactic acid bacteria isolated from meat stored in low temperature,which showed strong inhibitory activity against the indicated bacteria.The supernatant of this strain could still inhibit the growth of indicator strain strongly after eliminating hydrogen peroxide and organic acid.The inhibitory activity decreased sharply after treatment with pepsin,which indicated that this inhibitory material obtained the feature of protein;it could be named as one kind of bacteriocin.The test of bacterial inhibition spectrum showed that this was a kind of bacteriocin with wide inhibition spectrum.It was identified as Lactobacillus oris through detection of its appearance,physiological and biochemical characteristics.