Optimization of Fermentation Conditions for Extracellular Polysaccharide Production of Klebsiella

[Objective] The medium compositions and environmental conditions were optimized in order to improve the yield of extracellular polysaccharide(EPS) of Klebsiella.[Method] The single factor experiment and the orthogonal test were used to optimize the fermentation conditions.[Result] The results were showed as follows: 10×salt solution(containing Na2HPO4 100.00 g/L,FeSO4·7H2O 0.01 g/L,MgSO4·7H2O 2.00 g/L,CaCl2·2H2O 0.01 g/L,KH2PO4 30.00 g/L,K2SO4 10.00 and NaCl 10.00 g/L),glucose 30.00 g/L,peptone 0.50 g/L,beef extract 0.50 g/L and oleinic acid 1.50 g/L;the initial pH 7.0,culture temperature 24 ℃,inoculation size 10%,load volume of broth 200 ml/500 ml,and incubation time for 50 h.After the optimization,the yield of extracellular polysaccharide reached 6.70 g/L.[Conclusion] The study provides a technological basis for the batch production of bio-oligosaccharides in future.