Study on the immobilization of polyphenol oxidase of mulberry leaf

Kinetic characteristics of polyphenol oxidase(PPO)in mulberry leaf were studied. The result showed that the optimum pH and temperature was 7.0 and 40℃,respectively,and the Michaelisconstant was 0.093mol/L. The immobilization of PPO from mulberry leaf was researched. The activity of immobilized PPO reached its maximum when 4% sodium alginate,0.2% glutaraldehyde,0.2mol/L CaCl2 were used to immobilize the PPO,in an immobilization time of 2h and crosslinking time of 4h. The optimum pH and temperature was 6.0,and 50℃ respectively,and the enzyme became less susceptible to the temperature,the temperature did little affect to the enzymatic activity.