SECRETED EXPRESSION OF TRICHODERMA REESEI ENDO-β-GLUCANASE II GENE IN PICHIA PASTORIS AND ANYLYSIS OF ENZYMIC PROPERTIES

In this study an exons connecting method was used to amplify the complete sequence encoding the mature peptide of endo-1,4-glucanase II of Trichoderma reesei QM9414. The gene was inserted into the Pichia pastoris vector pPIC9K, downstream of α-factor signal peptide sequence. The resultant recombinant plasmid pQY2025 was lineared by BglII digestion and introduced into the host Pichia pastoris GS115 by electroporation. After screen, the recombinant P. pastoris strain Gp2025 was obtained and fermented in 25mL methylotrophic culture medium. The recombinant endoglucanase II activity could reach to 1573.0 u/mL. The properties of the recombinant endoglucanase II were characterized.