Comparison of two methods to detect the angiotensiongen gene M235T polymorphism

Objetive To compare the mutagenically separated allele-specific polymerase chain reaction technique (MS-PCR)and polymerase chain reaction-restriction fragment length polymorphism methods(PCR-RFLP) used to detect the angiotensinogen gene M235T polymorphism. Methods MS-PCR and PCR-RFLP were used to detect the distribution of genotype and allele frequency of angiotensinogen gene in 82 patients, and the gene polymorphism results by two different methods were compared. Also, the T allele frequency detected by two methods would be compared with the total frequency in local population. Results Compared with the results (T allele frequency 0.87) detected by MS-PCR, the distribution of genotype and allele frequency (T allele frequency 0.71) detected by PCR-RFLP showed significant difference(P0.01). The allele frequency detected by PCR-RFLP was accord with that of population in Asia and our country (0.63-0.79; 0.63-0.82).Conclusion Traditional PCR-RLFP used to detect angiotensiongen gene polymorphism is more reliable.