ScholarLens Back to search
2008•Chinese Journal of Aesthetic MedicineRequires access

Effects of intense pulsed light on collagens in rat skin

Xie Kuang-cheng

Open publisher page 0 citations

Abstract

Objective To study the effects of intense pulsed light(IPL) on collagens and mRNA expression of collagenⅠ,collagen Ⅲ in rat skin,and elucidate the mechanism of IPL.Methods 24 SD rats were irradiated on their left sides of the back with IPL,and their right sides were selected as control areas.We divided rats into four groups by time of irradiation.Skin specimens were taken both from treated and control areas after 1 week,2 weeks,4weeks and 8 weeks IPL treatments.Histological changes were observed by hematoxylin-eosin-staining,Masson trichrome staining,and mRNA expression levels of collagenⅠand collagen Ⅲ were detected by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR) .Results No obvious changes were seen at 1 week,2 weeks.At week 4 and week 8,the staining of collagen was stronger in treated areas than in control areas,and the mRNA expression levels of collagens were also increased at week 4 and week 8(P0.05) .Conclusion IPL can increase collagen production in rat skin.

About this research paper

What this paper is about

Objective To study the effects of intense pulsed light(IPL) on collagens and mRNA expression of collagenⅠ,collagen Ⅲ in rat skin,and elucidate the mechanism of IPL.Methods 24 SD rats were irradiated on their left sides of the back with IPL,and their right sides were selected as control areas.We divided rats into four groups by time of irradiation.Skin specimens were taken both from treated and control areas after 1 week,2 weeks,4weeks and 8 weeks IPL treatments.Histological changes were observed by hematoxylin-eosin-staining,Masson trichrome staining,and mRNA expression levels of collagenⅠand collagen Ⅲ were detected by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR) .Results No obvious changes were seen at 1 week,2 weeks.At week 4 and week 8,the staining of collagen was stronger in treated areas than in control areas,and the mRNA expression levels of collagens were also increased at week 4 and week 8(P0.05) .Conclusion IPL can increase collagen production in rat skin.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To study the effects of intense pulsed light(IPL) on collagens and mRNA expression of collagenⅠ,collagen Ⅲ in rat skin,and elucidate the mechanism of IPL.Methods 24 SD rats were irradiated on their left sides of the back with IPL,and their right sides were selected as control areas.We divided rats into four groups by time of irradiation.Skin specimens were taken both from treated and control areas after 1 week,2 weeks,4weeks and 8 weeks IPL treatments.Histological changes were observed by hematoxylin-eosin-staining,Masson trichrome staining,and mRNA expression levels of collagenⅠand collagen Ⅲ were detected by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR) .Results No obvious changes were seen at 1 week,2 weeks.At week 4 and week 8,the staining of collagen was stronger in treated areas than in control areas,and the mRNA expression levels of collagens were also increased at week 4 and week 8(P0.05) .Conclusion IPL can increase collagen production in rat skin.

Key concepts: Masson's trichrome stain, Staining, H&E stain, Intense pulsed light, Real-time polymerase chain reaction, Reverse transcription polymerase chain reaction, Messenger RNA, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effects of intense pulsed light on collagens in rat skin — Research Paper | ScholarLens