2005•Chinese Journal of Hospital PharmacyRequires access

Determination of gallic acid in Punica granatum L.extraction by HPLC

Benhong Zhou, Chun Liu, Zhenghua Wu, LI Xiao-jun, Jie Zhang

Abstract

OBJECTIVE A method for the determination of gallic acid in Punica granatum L extraction by means of RP-HPLC was established.METHODS HPLC condition: Zobax SB-C_(18)((250 mm)×(4.6 mm),(5 μm)),temperature of column is 40℃,use CH_(3)OH,EtOAc,C_(2)H_(5)OH,KH_(2)PO_(4),H_(3)PO_(4)gradient elution,280 nm as detective wave length.RESULTS the linear range of gallic acid is(0.062 1)-(0.414 0μg),r=(0.999 9).The average recovery was (100.14%),RSD was(1.99%).The content of gallic acid was(9.28)-(9.52 mg)·g~(-1) in 3 batches of Punica granatum L.CONCLUSION This method is precise and credible,it can apply to the quality control of Punica granatum L.

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Key concepts: Punica, Gallic acid, Chromatography, Chemistry, High-performance liquid chromatography, Extraction (chemistry), Gradient elution, Traditional medicine