The intervention of insulin-like growth factor on neonatal rats with hypoxic ischemic brain damage

Objective:To study the effect of exogenous insulin-like growth factor-1(IGF-1) on the levels of superoxide dismutase(SOD) and nitric oxide(NO) in brain tissues of neonatal rats with hypoxic ischemic brain damage(HIBD),explore the mechanism of IGF-1 in treatment of HIBD.Methods:75 7-day old Wistar rats were divided into different groups randomly: ①normal group(15 rats): without intervention;②HIBD model group(15 rats): left arteria carotis communis was ligated after anesthesia,then the rats were put into a 37℃ closed container after one hour,the rats were treated with inhalation of 0.08 oxygen of air for 2 hours,HIBD models were prepared;③normal saline control group(15 rats): the method to prepare HIBD models was similar to HIBD model group,then the rats were treated with intraperitoneal injection of normal saline(15 ml/kg);④salvia intervention group(15 rats): the method to prepare HIBD models was similar to HIBD model group,then the rats were treated with intraperitoneal injection of salvia injection(1.5 g/kg);⑤IGF-1 intervention group(15 rats): the method to prepare HIBD models was similar to HIBD model group,then the rats were treated with intraperitoneal injection of IGF-1(1.5 μg/kg).The rats were killed at 72 hours after hypoxia and ischemia,the cerebral cortex was obtained to prepare brain tissue homogenate;the levels of SOD and NO in brain tissues were detected.Results:The levels of SOD in HIBD model group and normal saline control group were lower than that in normal group,the levels of NO in HIBD model group and normal saline control group were higher than that in normal group,there was significant difference(P0.01);the levels of SOD in IGF-1 intervention group and salvia intervention group were higher than that in HIBD model group,the levels of NO in IGF-1 intervention group and salvia intervention group were lower than that in HIBD model group,there was significant difference(P0.01).Conclusion:IGF-1 can promote the increase of SOD level and the decrease of NO in cerebral cortex,which can prevent and treat HIBD.